Nermien E Waly
Helwan University, Egypt
Title: IL-10 and TGFβ meditates chondroprotective effect of glucosamine in rat model of osteoarthritis
Biography
Biography: Nermien E Waly
Abstract
Osteoarthritis (OA) is a complex disease of the whole joint. Glucosamine (GlcN) treatment may have a chondroprotective effect on OA. We investigate the mechanism of action of glucosamine treatment through Interleukin-10 (Il-10) and transforming growth factor β-1 (TGF β-1). Sixty-three male albino rats were used. A single intra-articular (i.a.) injection of 2 mg of Monosodium Iodoacetate (MIA) was injected into the knee joint of anesthetized rats. GlcN (50 and 100 mg/kg/day, p.o. for 2 month) was administered orally. Serum Il-10 and TGF-β1 were determined by ELISA. Histopathological changes in affected joints were examined. Serum level of IL-10 was significantly decreased in OA group compared to the control group (P value < 0.0001). On the other hand, the mean serum level of IL-10 was significantly increased in GlcN treated groups when compared to OA group (P value < 0.0001). Serum level of TGF β-1 was significantly elevated in OA group compared to the control group (P value < 0.0001). On the other hand, the mean serum level of TGF β-1 was significantly decreased in GlcN treated groups when compared to OA group (P value < 0.0001). Histopathological evaluation of Glucosamine treated groups showed different grades of healing according to Osteoarthritis Research Society International (OARSI) grading system. Our results show that GlcN exerts its chondroprotective effects via the stimulation of IL-10 production and Inhibition TGFβ1. Both Serum biomarkers can be a used as a tool for the follow up of articular cartilage damage in the clinical setting.